Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Receptor tyrosine kinase-like orphan receptor 1 inhibitor strictinin exhibits anti-cancer properties against highly aggressive androgen-independent prostate cancer

doi: 10.37349/etat.2023.00192

Figure Lengend Snippet: ROR1 and the downstream oncogenic signaling pathway are affected by strictinin. (A) Illustration of active ROR1 pathway. ROR1 leads to increased p-AKT and p-GSK3β, which promotes downstream oncogenic signaling pathways; (B) modulation of the ROR1 pathway by strictinin in PC3 cells. ROR1 inhibition diminishes p-AKT and p-GSK3β, which increases apoptosis and represses cell cycle progression, invasion, and migration in PC3 cells; (C) modulation of the ROR1 pathway by strictinin in DU145 cells. ROR1 inhibition diminishes p-AKT and strictinin increases the phosphorylation of GSK3β, which is likely a contributing molecular mechanism by which strictinin suppresses the migration and invasion of DU145 cells. The figure was created with Biorender.com

Article Snippet: ROR1 (#16540S, Cell signaling, USA); AKT (#9272S, Cell signaling, USA); phosphorylated-AKT-ser473 [p-AKT-ser473 (#4060S, Cell signaling, USA)]; GSK3β (#12456S, Cell signaling, USA); p-GSK3β-ser9 (#5558S, Cell signaling, USA); Twist1 (#69366S, Cell signaling, USA); matrix metalloproteinase 9 (MMP9, #13667S, Cell signaling, USA); Snail (#3895S, Cell signaling, USA); glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #C2819, scbt, USA); HRP conjugated Goat anti-rabbit secondary (#7074S, Cell signaling, USA); HRP conjugated Goat anti-mouse secondary (#1706516, Biorad, USA).

Techniques: Protein-Protein interactions, Inhibition, Migration, Phospho-proteomics

Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Receptor tyrosine kinase-like orphan receptor 1 inhibitor strictinin exhibits anti-cancer properties against highly aggressive androgen-independent prostate cancer

doi: 10.37349/etat.2023.00192

Figure Lengend Snippet: Strictinin inhibits ROR1-mediated oncogenic signaling in PC3 cells. (A) Immunoblot on PC3 cells treated with control, 250 µmol/L strictinin for 24 h, or 250 µmol/L strictinin for 48 h. ROR1 is visualized; (B) quantification of ROR1 protein band normalized to GAPDH. Data are represented as the mean ± standard error of the mean (SEM) from three independent western blot experiments; (C) immunoblot on PC3 cells treated with control, 250 µmol/L strictinin for 24 h, or 250 µmol/L strictinin for 48 h. AKT, p-AKT-ser473, GSK3β, p-GSK3β-ser9 visualized; (D) quantification of p-AKT/AKT and p-GSK3β/GSK3β protein bands normalized to GAPDH. Data are represented as the mean ± SEM from three independent western blot experiments. N = 3. ** P value < 0.01; * P value < 0.05

Article Snippet: ROR1 (#16540S, Cell signaling, USA); AKT (#9272S, Cell signaling, USA); phosphorylated-AKT-ser473 [p-AKT-ser473 (#4060S, Cell signaling, USA)]; GSK3β (#12456S, Cell signaling, USA); p-GSK3β-ser9 (#5558S, Cell signaling, USA); Twist1 (#69366S, Cell signaling, USA); matrix metalloproteinase 9 (MMP9, #13667S, Cell signaling, USA); Snail (#3895S, Cell signaling, USA); glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #C2819, scbt, USA); HRP conjugated Goat anti-rabbit secondary (#7074S, Cell signaling, USA); HRP conjugated Goat anti-mouse secondary (#1706516, Biorad, USA).

Techniques: Western Blot, Control

Journal: Exploration of Targeted Anti-tumor Therapy

Article Title: Receptor tyrosine kinase-like orphan receptor 1 inhibitor strictinin exhibits anti-cancer properties against highly aggressive androgen-independent prostate cancer

doi: 10.37349/etat.2023.00192

Figure Lengend Snippet: Strictinin modulates ROR1-mediated AKT-GSK3β signaling in DU145 cells. (A) Immunoblot on DU145 cells treated with control, 250 µmol/L strictinin for 24 h, or 250 µmol/L strictinin for 48 h. ROR1 is visualized; (B) quantification of ROR1 protein band normalized to GAPDH. Data are represented as the mean ± SEM from three independent western blot experiments; (C) immunoblot on DU145 cells treated with control, 250 µmol/L strictinin for 24 h, or 250 µmol/L strictinin for 48 h. AKT, p-AKT-ser473, GSK3β, p-GSK3β-ser9 visualized; (D) quantification of p-AKT/AKT and p-GSK3β/GSK3β protein bands normalized to GAPDH. Data are represented as the mean ± SEM from three independent western blot experiments. N = 3. ** P value < 0.01; * P value < 0.05

Article Snippet: ROR1 (#16540S, Cell signaling, USA); AKT (#9272S, Cell signaling, USA); phosphorylated-AKT-ser473 [p-AKT-ser473 (#4060S, Cell signaling, USA)]; GSK3β (#12456S, Cell signaling, USA); p-GSK3β-ser9 (#5558S, Cell signaling, USA); Twist1 (#69366S, Cell signaling, USA); matrix metalloproteinase 9 (MMP9, #13667S, Cell signaling, USA); Snail (#3895S, Cell signaling, USA); glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #C2819, scbt, USA); HRP conjugated Goat anti-rabbit secondary (#7074S, Cell signaling, USA); HRP conjugated Goat anti-mouse secondary (#1706516, Biorad, USA).

Techniques: Western Blot, Control